A simple bioanalytical method was developed to estimate Carbamazepine from human plasma with due consideration of cost effective sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of carbamazepine and Carbamazepine D10 using as internal standard(IS) in K2EDTA human plasma. Both the analytes were extracted by protein precipitation technique. The chromatographic separation was performed by isocratic on Inert sustain C18 100*, 4.6 mm, 5 µ with a mobile phase of 0.1% glacial acetic acid solution in water: organic mixture (35:65) (v/v) [organic mixture-methanol:acetonitrile (80:20)] followed by detection using mass spectrometry. The protonated analyte was quantitated in positive ionization by multiple reactions monitoring with a mass spectrometer. The method was validated and the lower limit of quantification for Carbamazepine and Carbamazepine D10 was found to be 50 ngmL−1 and 50 ngmL−1 respectively. The mean recovery for Carbamazepine and Carbamazepine D10 ranged from 89.34 to 98.01% and also this method no significant matrix effect on analytes. The method was validated over the concentration range of 50 ng/mL to 8000 ng/mL for both Carbamazepine and Carbamazepine D10 in human plasma with a accuracy of within run and between run in the range of 80-120%. The within run and between run precision of Carbamazepine and Carbamazepine D10 were also within the range of 20% (for LLOQ level) and 15% ( for other than LLOQ) of % CV.
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